Fluorophore¶

public interface Fluorophore¶

A single fluorophore including its position and photophysical properties.

Author:	Kyle M. Douglass

Methods¶

public boolean isBleached()¶

Has the fluorophore been bleached? If so, it can never return to a fluorescence-emitting state.

Returns:	A true/false value describing whether the fluorophore is bleached.

public boolean isOn()¶

Describes whether the fluorophore is emitting light or is in a dark state.

Returns:	A true/false value describing whether the fluorophore is emitting.

public void recalculateLifetimes(double laserPower)¶

This method recalculates the lifetimes of the fluorophore’s state system based on the laser power.

Parameters:	laserPower – The new power of the laser.