GibsonLanniPSF¶
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public final class
GibsonLanniPSF
implements PSF¶ Computes an emitter PSF based on the Gibson-Lanni model. This algorithm was first described in Li, J., Xue, F., and Blu, T. (2017). Fast and accurate three-dimensional point spread function computation for fluorescence microscopy. JOSA A, 34(6), 1029-1034. The code is adapted from MicroscPSF-ImageJ by Jizhou Li: https://github.com/hijizhou/MicroscPSF-ImageJ
Author: Kyle M. Douglass
Methods¶
generatePixelSignature¶
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public double
generatePixelSignature
(int pixelX, int pixelY)¶ Computes the relative probability of receiving a photon at pixel (pixelX, pixelY) from an emitter at (emitterX, emitterY, emitterZ).
Parameters: - pixelX – The pixel’s x-position.
- pixelY – The pixel’s y-position.
Returns: The probability of a photon hitting this pixel.
generateSignature¶
getRadius¶
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public double
getRadius
()¶ Computes the half-width of the PSF for determining which pixels contribute to the emitter signal. This number is based on the greatest horizontal or vertical extent of the grid that the PSF is computed on. If maxRadius is smaller than that determined by the PSF’s computational grid, then maxRadius is returned.
Returns: The width of the PSF.