PSFBuilder¶
-
public interface
PSFBuilder¶ Defines the Builder interface for constructing PSFs. Passing Builders instances, rather than PSF instances, to the simulation allows the PSF to be constructed at different times during the simulation. For example, one might set basic parameters like the wavelength in the beginning of the simulation and set the emitter’s z-position immediately before a frame is computed. This means the simulation can dynamically create new PSF instances in response to changing simulation parameters.
Author: Kyle M. Douglass
Methods¶
FWHM¶
-
public PSFBuilder
FWHM(double FWHM)¶ The Gaussian approximation’s FWHM for this PSF.
NA¶
-
public PSFBuilder
NA(double NA)¶ The numerical aperture of the objective.
eX¶
-
public PSFBuilder
eX(double eX)¶ Sets the emitter’s x-position.
Parameters: - eX – The emitter’s x-position. [pixels]
eY¶
-
public PSFBuilder
eY(double eY)¶ Sets the emitter’s y-position.
Parameters: - eY – The emitter’s y-position. [pixels]
eZ¶
-
public PSFBuilder
eZ(double eZ)¶ Sets the emitter’s z-position.
Parameters: - eZ – The emitter’s z-position. [pixels]
resLateral¶
-
public PSFBuilder
resLateral(double resLateral)¶ Object space pixel size
stageDisplacement¶
-
public PSFBuilder
stageDisplacement(double stageDisplacement)¶ Sets the stage displacement for axially-dependent PSFs.
wavelength¶
-
public PSFBuilder
wavelength(double wavelength)¶ Wavelength of the light.