PalmDynamics.Builder

public static class Builder implements FluorophoreDynamicsBuilder

Builder for creating PALM dynamical systems.

Methods

build

public PalmDynamics build()

Initialize a PALM-like dynamical system for fluorescence dynamics.

Returns:The PALM dynamical system.

kA

public Builder kA(double kA)

The activation rate

Parameters:

• kA –

kB

public Builder kB(double kB)

The bleaching rate

kD1

public Builder kD1(double kD1)

The rate of entering the first dark state

kD2

public Builder kD2(double kD2)

The rate of entering the second dark state

kR1

public Builder kR1(double kR1)

The return rate from the first dark state

kR2

public Builder kR2(double kR2)

The return rate from the second dark state

signal

public Builder signal(double signal)

The average number of photons per fluorophore per frame

Parameters:

• signal –

Returns:

PalmDynamics builder

wavelength

public Builder wavelength(double wavelength)

The center wavelength of the fluorescence emission

Parameters:

• wavelength –

Returns:

PalmDynamics builder